Nucleic Acid Mwepụta Ma ọ bụ Ngwa Ọcha
Nucleic Acid Emmịpụta Or Ịdị ọcha Kitma ọ bụ echekwara na -20 ℃. Ekwesịrị ibugharị ihe nlele ahụ site na iji curling 0 ℃.
Introduction
Akpụpụta Nucleic Acid Ma ọ bụ Ngwa Ọcha (Ụzọ Magnetic Beads) ka emebere maka nchacha akpaaka nke RNA na DNA sitere na mmiri ahụ (dị ka swabs, plasma, serum) site na iji ngwa mmịpụta nucleic acid na-akpaghị aka. Nkà na ụzụ magnetik na-enye DNA/RNA dị elu nke kwesịrị ekwesị maka ojiji ozugbo na ngwa mgbada dị ka mmụba ma ọ bụ mmeghachi omume enzymatic ndị ọzọ.
Application Range
A ghasara ọbara niile, plasma, serum na ihe nlele anụ ahụ ndị ọzọ ozugbo wee gbarie ya. Ngwa ngwa magnetik nanometer super paramagnetic na-ahọpụtara acid nucleic a tọhapụrụ. Mgbe ahụ, a na-ewepụ protein, ion nnu inorganic na ihe ndị na-adịghị ọcha site na ngwọta ịsacha.
Kit Contents
| Nwamba. Mba. | YXN-VIRAL01-32A-BR | Ngwa | ||
| -50A | - 100A | |||
| Nha | 32 Tes | 50Nnwale | 100Nnwale | |
| Lysis Buffer | 96 eburu ụzọ mkpọ ed Efere 2 iberibe | 25ml | 50ml | Surfactant na Tris |
| Ebe nchekwa ihe I | ★15ml | ★30ml | Ngwọta nnu dị elu | |
| Sachaa ihe nchekwa Il | ★6ml*2 | ★12ml*2 | Ngwọta nnu dị ala | |
| Ihe nchekwa ihe | 10ml | 20ml | Ngwọta nnu dị ala | |
| MagaBio Reagent | 1.0ml | 2.0ml | Ihe magnetik | |
| Akwụkwọ ntuziaka (=YXN-VIRAL01-32A-BR) | 1 | 1 | 1 | |
| Nihe:MakaYXN-VIRAL01-32A-BR-50A,tinye 15mL zuru oke ethanol ka ★15mL Wash Buffer I tupu eji; tinye 24mL Absolute ethanol na ★6mL Wash buffer Il tupu eji. | ||||
| MakaYXN-VIRAL01-32A-BR-100A, tinye 30mL Absolute ethanol ka ★ 30mL Wash Buffer I tupu eji; tinye 48mL Absolute ethanol na ★12mL Wash buffer Il tupu eji ya.【Reagents nke onye ọrụ ga-akwado ya】Biko dozie ethanol zuru oke (ọkwa nyocha) n'onwe gị. | ||||
Storage Conditions
Mgbe ọ bịarutere ngwa ahụ, a ga-echekwa ihe ndị mejupụtara ngwa n'ime ụlọ okpomọkụ (15 - 25 Celsius C). Ndị reagents kwụsiri ike ruo otu afọ site na ụbọchị nrụpụta.
Sample Requirements
1. Ọdabara sample: Swabs, plasma, serum na dum ọbara wdg.
2. Nlereanya nchekwa na njem njem: Ekwesịrị ịnwale nlele ozugbo
Materials na Devices Required but Not Provided
1. Ntufu ntụ ntụ na-enweghị aka
2. Akpa biohazard
3. Pensụl ma ọ bụ kwa
Procedure
Ndị na-esonụ na-eji sample mmịpụta swab ude mmiri dị ka ihe atụ iji kọwaa nkenke usoro usoro nke mmịpụta reagent n'ime ngwa mmịpụta nucleic acid.Bioer NPA-32Pma ọ bụSMART 32. Maka ụdị nlele ndị ọzọ, biko rụtụ aka na ntuziaka onye ọrụ. Ndị ahịa nwekwara ike rụọ ya dịka nnweta nnwale:
1. Reagent Nkwadebe
a. MakaYXN-VB03-32A-50A na YXN-VB03-32A-100A
Tinye 500uL Lysis Buffer na kọlụm 1 na 7 nke efere 2.2mL 96-miri miri emi, 500uL Wash Buffer I na kọlụm 2 na 8, 500uL Wash Buffer II na kọlụm 3, 4 na 9,10; 70uL Elution Buffer na kọlụm 5 na 11, 180uL mmiri dị ọcha na 20uL MagaBio Reagent na kọlụm 6 na 12 (a ga-agwakọta bead magnet nke ọma tupu ejiri ya),
b. MakaYXN-VB03-32A
Tinye reagents 96 nke etinyegoro nke ọma na ụlọ okpomọkụ. Gbanyụọ efere 96 nke ọma ihu ugboro atọ, ma dọkasịa akpa rọba ahụ. Centrifuge reagenti eburula ụzọ maka sekọnd ole na ole (ma ọ bụ jiri aka na-efegharị ugboro ole na ole) ka ịzenarị reagent ịrapara na mgbidi nke tubes. Gbanyụọ ihe nkiri aluminom nke efere 96-well ma chọpụta ntụzịaka nke efere ahụ (ihe ndọta ndọta na kọlụm #6 na #12),
2.Ihe atụ mmịpụta
1. Tinye ihe nlele 300uL na ogidi efere 96 nke ọma # 1 na # 7, biko zere mmetọ obe,
2. Debe efere 96 miri emi nke ọma na ngwá ọrụ ahụ, wụnye ndụmọdụ 8-strip na ngwá ọrụ ahụ,
3. Gbaa mmemme ahụ dịka usoro ndị a si dị,
4. Mgbe emechara nchacha akpaka, nyefee elution Buffer na ogidi 5 na 11 gaa na tube centrifuge 0.5mL antinuclear dị ọcha; Ọ bụrụ na ejighị ya ozugbo, biko chekwaa na-20 Celsius.
Performance Characteristics
1. A na-achọpụta ngwaahịa a amịpụtara site na nhụta ihe nchọpụta HBV DNA dị elu iji ruo nhụsianya nke 10 IU/ml. A na-achọpụta ngwaahịa a amịpụtara site na reagent nchọpụta HCV RNA nwere mmetụta dị elu iji ruo nghọta nke 50 IU/ml.
2. Họrọ 4 samples (serum / Plasma sample, nasopharyngeal swab sample, cervical exfoliated cell sample), onye ọ bụla sample na-diluted 10 ugboro na 3 gradients (gụnyere mbụ sample mkpokọta nke 4 mkpokọta), na-eji ruru eru reagents na ule na-elekọta mmadụ ịchọpụta esịtidem. usoro ntụaka dị ka ntuziaka ngwaahịa si dị, yana uru Ct nke batch ọ bụla dị iche na ihe na-erughị 1.
| Step | Ọfọn Ebe | Program Aha | Waiting Time(min:SS) | Mixing Time(mna:SS) | Magnet Time(min:SS) | Adsorption | Speed | Volume tatus (μL) | Teze ukwu |
| 1 | 1 | Lynwanne | 0:00 | 2:00 | 0:00 | F | 700 | 80 | |
| 2 | 6 | Bntị | 0:00 | 0:15 | 0:15 | √ | F | 200 | |
| 3 | 1 | Bind | 0:00 | 3:00 | 0:45 | √ | F | 700 | |
| 4 | 2 | Wash1 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 5 | 3 | Wash2 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 6 | 4 | Wash3 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 7 | 5 | Elution | 2:00 | 2:30 | 0:30 | F | 70 | 80 | |
| 8 | 6 | Tufuo | 0:00 | 0:15 | 0:00 | F | 200 |
Safety
1. GENERAL Nchekwa.
Iji ngwaahịa a n'ụzọ akọwapụtaghị n'akwụkwọ onye ọrụ nwere ike bute mmerụ ahụ nkeonwe ma ọ bụ mebie ngwa ma ọ bụ ngwaọrụ. Gbaa mbọ hụ na onye ọ bụla na-eji ngwaahịa a anatala ntuziaka na omume nchekwa izugbe maka ụlọ nyocha yana ozi nchekwa enyere n'ime akwụkwọ a.
1.1 Tupu iji ngwa ma ọ bụ ngwaọrụ, gụọ ma ghọta ozi nchekwa enyere na akwụkwọ onye ọrụ nke onye nrụpụta ngwa ma ọ bụ ngwaọrụ nyere.
1.2 Tupu ijikwa kemịkalụ, gụọ ma ghọta akwụkwọ nchekwa data nchekwa (SDS) niile ma jiri ngwa nchekwa onwe nke dabara adaba (gloves, gown, mkpuchi anya, wdg). Iji nweta SDS, hụ ngalaba "Akwụkwọ na Nkwado" n'ime akwụkwọ a.
2. Chemical nchekwa
Ijikwa kemịkalụ izugbe. Iji belata ihe egwu, hụ na ndị ọrụ ụlọ nyocha na-agụ ma na-eme ụkpụrụ nchekwa nchekwa izugbe maka ojiji kemịkalụ, nchekwa, na mkpofu enyere n'okpuru, wee gakwuru SDS dị mkpa maka akpachapụ anya na ntuziaka akọwapụtara: Gụọ ma ghọta akwụkwọ nchekwa data nchekwa (SDS) nke kemịkalụ nyere. emeputa tupu ị chekwaa, jikwaa, ma ọ bụ rụọ ọrụ na kemịkalụ ọ bụla ma ọ bụ ihe dị ize ndụ.
2.1 Wedata kọntaktị na kemịkalụ. Yiri ngwa nchedo onwe gị nke ọma mgbe ị na-eji kemịkalụ (dịka ọmụmaatụ, enyo nchekwa, uwe aka ma ọ bụ uwe nchebe).
2.2 Wedata iku ume nke kemịkalụ. Ahapụla akpa kemịkalụ ghe oghe. Jiri naanị na ikuku ikuku zuru oke (dịka ọmụmaatụ, mkpuchi uzuzu).
2.3 Na-enyocha mgbe niile maka mwepu kemịkal ma ọ bụ mwụfu. Ọ bụrụ na ntapu ma ọ bụ mwụfu apụta, soro usoro nchacha nke onye nrụpụta ka akwadoro na SDS.
2.4 Jikwaa ihe mkpofu kemịkalụ n'ime mkpuchi anwụrụ ọkụ.
2.5 Gbaa mbọ hụ na a na-eji ihe mkpofu mbụ na nke abụọ. (Ihe mkpofu mbụ
akpa na-ejide ihe mkpofu ozugbo. Akpa nke abụọ nwere ihe mwụfu ma ọ bụ ntapu sitere na akpa bụ isi. Akpa abụọ a ga-adakọrịrị na ihe mkpofu wee zute ihe gọọmentị etiti, steeti na mpaghara chọrọ maka nchekwa akpa.).
2.6 Mgbe ikpochapu ihe mkpofu, mechie ya na okpu enyere.
2.7 Kọwapụta (site na nyocha ma ọ bụrụ na ọ dị mkpa) ihe mkpofu nke ngwa, reagents na ihe ndị a na-eji eme ihe na ụlọ nyocha gị.
2.8 Gbaa mbọ hụ na echekwara, bufee, bufee ma kpofuo ihe mkpofu dịka iwu mpaghara, steeti/ógbè na/ma ọ bụ obodo niile siri dị.
2.9 Ihe redioaktivu ma ọ bụ ihe ndị dị ndụ nwere ike ịchọ njikwa pụrụ iche, yana oke mkpofu nwere ike itinye.
3.Ndụ ndụ ihe egwu nchekwa
Biohazard nwere ike ime. Dabere na ihe nlele ejiri na ngwa a, elu nwere ike were dị ka ihe ize ndụ bio. Jiri ụzọ mkpochapụ kwesịrị ekwesị mgbe ị na-eji ihe ize ndụ na-arụ ọrụ.
BIOHAZARD. Nlere nke ihe ndị dị ndụ dị ka anụ ahụ, mmiri ara, ihe na-efe efe, na ọbara mmadụ na anụmanụ ndị ọzọ nwere ike ibunye ọrịa na-efe efe. Soro usoro mpaghara, steeti/mpaghara, na/ma ọ bụ iwu obodo niile dị. Yiri ngwa nchebe kwesịrị ekwesị, nke gụnyere ma ọnweghị oke na: anya mkpuchi, ọta ihu, uwe/ uwe nyocha, na uwe aka. Ekwesịrị ịrụ ọrụ niile n'ụlọ arụnyere nke ọma site na iji ngwa nchekwa kwesịrị ekwesị (dịka ọmụmaatụ, ngwaọrụ njide anụ ahụ). Ekwesịrị ịzụ ndị mmadụ n'otu n'otu dịka usoro iwu na ụlọ ọrụ/ụlọ ọrụ chọrọ tupu ha ejiri ihe nwere ike ibute ọrịa.
Gụọ ma soro ntuziaka dị na/ma ọ bụ usoro iwu chọrọ na ndị a:
Na US: ntuziaka Ngalaba Ahụike na Ọrụ mmadụ nke US bipụtara na Biosafety in Microbiological and Biomedical Laboratories achọtara na: www.cdc.gov/biosafety.
Ụkpụrụ nchekwa na ahụike ọrụ, ọrịa na-ebute ọbara (29 CFR§1910.1030), achọtara na:
www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html
Usoro mmemme Biosafety nke ụlọ ọrụ / ụlọ ọrụ gị maka iji/ijikwa ihe ndị nwere ike ibute ọrịa. Ozi nkowa mgbakwunye gbasara ntuziaka biohazard dị na: www.cdc.gov.
Na EU: Lelee ntuziaka mpaghara na iwu gbasara nchekwa biohazard na biosafety wee rụtụ aka na omume kachasị mma e bipụtara na akwụkwọ ntuziaka ụlọ nyocha biosafety nke World Health Organisation (WHO), mbipụta nke atọ, achọtara na: www.who.int/csr/resources/publications / biosafety/WHO_CDS_CSR_LYO_200 4_11/am/.
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